• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    A form piece is disclosed which may be mounted in a test tube cuvette or similar vessel and is coated with an antibody, enzyme or other material to be used in laboratory tests. The form piece is preferably made in a ring-like configuration and includes projecting members which space the form piece from the sides of the vessel.
    公开号:SU791266A3
    申请号:SU782566403
    申请日:1978-01-13
    公开日:1980-12-23
    发明作者:А.Суованиеми Осмо；Экхольм Пертти；Суни Юкка；Ярнефельт Йохан
    申请人:Коммандииттинхтие Финнпипетте Осмо А.Суованиеми (Фирма)；
    IPC主号:
    专利说明:

    t
    This invention relates to medicine and relates to devices for immunoassay and enzyme reactions.
    A device for immunoassay is known which contains a container for a reagent and a device for fixing the test substance ij.
    However, the known device is difficult in its design.
    The purpose of the invention is to simplify the design of the device.
    This is achieved by the fact that in the proposed device for immunoassay, the device for fixing the test substance is made in the form of a ring having protrusions on the outer side surface to ensure contact with the inner surface of the reagent container.
    In this case, the device for fixing the test substance can be made of a porous material. In addition, the fixture for fixing the test substance can be made of several at least two layers of materials.
    FIG. 1 schematically shows an immunoassay device, longitudinal section; in fig. 2-5 - device for fixing the explorer
    substance cut; in fig. 6 — immunoassay device with two devices for fixing the test substance; in fig. 7 is a device for fixing the test substance, made of a porous material) in FIG. 8 is a device for fixing a test substance, made of several layers of materials in FIG. 9 is a device for fixing a test substance having a shell, a core and an intermediate layer, in FIG. 10 and 11 - open ring, longitudinal section and
    15 view from above; in fig. 12 shows a support plate with holes for the passage of a beam; FIG. 13 - containers for placing the ring, longitudinal section; in fig. 14 - tips for
    20 pipettes with a ring placed inside them.
    The device (Fig. 1) contains a container 1 for reagent 2 and a device for fixing the test substance, made in the form of a ring 3 (Fig. 2-5). Ring 3 is located in the tank 1 at a given height.
    The ring can have a different shape and configuration, and its surfaces can be smooth (Fig. 3). The outer and inner surfaces of the ring 3 may have a conical shape and be perpendicular to the base of the ring. To ensure contact with the inner surface of reagent tank 1, a ring 3 (Fig. 4) on the outer side surface has protrusions 4, and on the lower surface it has a protrusion 5 located around its circumference, while the protrusion 2 can be made as continuous as and discrete. The lower part of the protrusion 4 is made pointed, which prevents the lower part of the ring from sticking, for example to the bottom of the container. The ring (Fig. 5) has a core 6, a shell 7 and protrusions 8 on its lower surface. The core 6 and the shell 7 can be made of the same material and of different materials, while the core 6 can serve as a mechanical carrier and be made of magnetic iron and the shell 7 as a binding material. A magnet can be used to remove the ring from the container. The device is used for sampling and analysis as follows. Ring 3 is coated, for example, with antigens, antibodies or an enzyme. Enzyme-coated can be placed in a container, such as a cuvette or a plate with sockets. Ring 3 is then introduced into the residue, for example, on the basis of nasal or pharyngitis mucus, from which antigens on the ring select their corresponding antibodies. Next, the ring 3 is washed with an appropriate wash solution so that those antibodies that are contained in the mucus cannot separate from the ring. Then the analysis can be continued using known means. The ring is placed in tank 1, to which reagent 2 is added, which generally contains a buffer solution and an antibody enzyme corresponding to the antibodies tested. The reagent is added after the expiration of the incubation period and the washing process. The results of the study are judged by the color change of the reagent. More accurate results are obtained by examining the kinetics or measuring the content of the final components. Using the procedure described above, a quick diagnosis can be made, for example, of an infectious disease of the respiratory tract or urinary tract. Ring samples are easy to store and carry. Enzyme-coated rings can be reused many times since they are easily washed and stored. In the same container 9 (FIG. 6), two similar or different rings 10 and 11 can be placed simultaneously. The materials contained in reagent 12 can interact, for example, with materials placed on ring 10, and then on ring 11 with the inclination of the container 9. If the container is shaken, the materials of the reagent 12 react with materials that are located both on ring 10 and ring 11. On the vessel wall, in this way, one or more rings can be fixed at a given height, and on the cork 13 - one or more reacting. The fixture for fixing the test substance can be made of a porous material to increase its surface. The ring (FIG. 7) is provided with a porous material 14 and protrusions 15 located on its outer side and bottom surfaces. The projections 15 can be made in one piece with the ring, have a different shape and are made of any suitable material. Device for fixing the test substance can be made of several, at least two materials. FIG. 8 shows a ring made of three layers of 16-18 materials. The layers can be made of one and different materials, while they can be of any desired shape and applied in any given sequence. FIG. 9 shows a ring having a core 19, an intermediate layer 20 and a shell 21. The thickness and configuration of the layers can be changed. The functions of the various layers can vary, for example, nucleus 19 binds the enzyme, and the shell passes only molecules that are smaller than the enzyme molecules, in this case, the ring acts as an electrode. For example, for an enzyme. The rings are made so that between it and the reagent tank either there is a small gap, or the connection between the ring and the vessel wall is carried out by means of the projections 4. The ring can also be made in the form of an open ring (Figs. 10, 11), and the gap between the edges of the open ring can be changed as necessary. Such a ring may also have protrusions 22 on the outer or lower surface, consist of several layers and be placed in the container either rigidly or with a gap with its walls.
    The rings 23 (FIG. 12) can be connected to the supporting plate 24, having openings 25 for the passage of the rays, by means of rods 26 with the possibility of adjusting the arrangement of the rings 2, 3 relative to the plate 24.
    Reagent tanks may have a flat, concave and cone-shaped bottom 27, 28 and 29 (Fig. 13). In the nests placed rings 30.
    If, during measurement, the light passes vertically in a direction parallel to the vertical axis of the container, the ring in the latter is horizontal, while the light passes through the central opening of the ring, and if the light passes horizontally, first through one vertical wall of the container, then through a column of liquid and through the second vertical wall of the container, the ring is placed vertically in the latter, while the light also passes through the central opening in the ring.
    The ring can also be used in analyzers containing a centrifuge, while in the tank the ring is placed in a horizontal plane.
    The rods 26 (FIG. 12) may be magnets, and may be made of a non-magnetic material, while if the rings 23 contain iron, they interact with the magnetic rods 26.
    FIG. 14 shows the pipette tips 31, which can be made either removable or at the same time integral with the pipettes. A conical ring 32 can be placed inside each pipette 31, and a reaction can proceed inside the pipette tip.
    The ring 33 can be placed on the outer surface of the tip 31,
    which can also serve as a pebbler of the rings 33. Several rings can be placed inside the tip and on its outer surface.
    The proposed immunoassay device, simply by its design, improves accuracy, reproducibility, usability and speed of determination.
    By using the proposed device, the stability of the bond between enzymes and the ring is significantly increased. In particular, in the case of enzymes with an increase in thermal stability, higher reaction temperatures can be used, and the reaction rate also increases.
    权利要求:
    Claims (3)
    [1]
    1. An immunoassay device containing a reagent tank and a fixture for fixing the test substance, characterized in that, in order to simplify the construction, the fixture for fixing the test substance is made in the form of a ring having protrusions on the outer side surface to ensure contact with the inner surface of the tank for reagent.
    [2]
    2. The device according to claim 1, characterized in that the device is made of a porous material.
    [3]
    3. The device according to claim 1, characterized in that the device is made of several at least two layers of materials.
    Sources of information taken into account in the examination
    1. US patent 3,932,141, cl. G 01 N 33/16, 1976 (prototype).
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    同族专利:
    公开号 | 公开日
    PL203998A1|1978-08-28|
    US4147752A|1979-04-03|
    FI54842B|1978-11-30|
    DD133863A5|1979-01-24|
    SE7800236L|1978-07-15|
    FI54842C|1979-03-12|
    JPS5925461B2|1984-06-18|
    IT1109581B|1985-12-23|
    PL114120B1|1981-01-31|
    JPS53130422A|1978-11-14|
    FR2377629A1|1978-08-11|
    DK18178A|1978-07-15|
    CH637219A5|1983-07-15|
    DE2801026C2|1983-03-03|
    FI770116A|1978-07-15|
    FR2377629B1|1983-02-04|
    BE862908A|1978-07-13|
    IT7867058D0|1978-01-12|
    GB1571872A|1980-07-23|
    DE2801026A1|1978-07-20|
    SE444227B|1986-03-24|
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    FI770116A|FI54842C|1977-01-14|1977-01-14|FORMULATION OF THE IMMUNITATION FOR IMMUNITATION AND ENZYMATION|
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